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#methods

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Douglas E. Welch<p>How to Stop Thinking Too Much via Raptitude [Shared]</p><p>I appreciate Sam Harris’s apt analogy about inner monologues — being caught up in your own thinking is like having been kidnapped and held hostage by the most boring person on earth. You’re forced to listen, as though at gunpoint, to an internal commentator who insists on telling you its impressions of everything it notices or thinks about.</p><p>Nothing is too petty, too repetitive, or too obvious for the boring kidnapper’s ongoing monologue: Susan was wrong to criticize people who wear Crocs to the grocery store; a certain politician is the worst person alive and here’s why; your ex-partner was definitely out of line when he accused you of wasting dish detergent that time; the two halves of this Oreo don’t line up, but it would be so much nicer if they did.</p><p><a href="https://welchwrite.com/blog/2025/07/28/how-to-stop-thinking-too-much-via-raptitude-shared/" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">welchwrite.com/blog/2025/07/28</span><span class="invisible">/how-to-stop-thinking-too-much-via-raptitude-shared/</span></a></p><p><a href="https://nerdculture.de/tags/Thinking" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Thinking</span></a> <a href="https://nerdculture.de/tags/mind" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>mind</span></a> <a href="https://nerdculture.de/tags/overthinking" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>overthinking</span></a> <a href="https://nerdculture.de/tags/meditation" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>meditation</span></a> <a href="https://nerdculture.de/tags/settling" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>settling</span></a> <a href="https://nerdculture.de/tags/repetitive" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>repetitive</span></a> <a href="https://nerdculture.de/tags/methods" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>methods</span></a> <a href="https://nerdculture.de/tags/technique" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>technique</span></a> <a href="https://nerdculture.de/tags/shared" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>shared</span></a></p>
サファイア・ネオ<p>AI絵生成 Генерація зображень ШІ<br>AI絵生成ソフトで何処が失敗してるかと云えば、人間の体の構造や比率を充分学習させてないと云う点が大きい。<br>Основна помилка програмного забезпечення для створення зображень ШІ полягає в тому,</p><p><a href="https://note.com/poison_raika/n/n501ac31af2f1" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">note.com/poison_raika/n/n501ac</span><span class="invisible">31af2f1</span></a></p><p>&lt;&gt;</p><p><a href="https://mastodon.social/tags/AI" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>AI</span></a> <a href="https://mastodon.social/tags/3d" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>3d</span></a> <a href="https://mastodon.social/tags/image" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>image</span></a> <a href="https://mastodon.social/tags/model" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>model</span></a> <a href="https://mastodon.social/tags/new" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>new</span></a> <a href="https://mastodon.social/tags/human" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>human</span></a> <a href="https://mastodon.social/tags/software" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>software</span></a> <a href="https://mastodon.social/tags/animals" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>animals</span></a> <a href="https://mastodon.social/tags/traditional" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>traditional</span></a> <a href="https://mastodon.social/tags/layout" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>layout</span></a> <a href="https://mastodon.social/tags/structure" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>structure</span></a> <a href="https://mastodon.social/tags/generation" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>generation</span></a> <a href="https://mastodon.social/tags/frequency" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>frequency</span></a> <a href="https://mastodon.social/tags/necessary" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>necessary</span></a> <a href="https://mastodon.social/tags/develop" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>develop</span></a> <a href="https://mastodon.social/tags/vizualize" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>vizualize</span></a> <a href="https://mastodon.social/tags/objects" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>objects</span></a> <a href="https://mastodon.social/tags/dimensions" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>dimensions</span></a> <a href="https://mastodon.social/tags/approach" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>approach</span></a> <a href="https://mastodon.social/tags/methods" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>methods</span></a> <a href="https://mastodon.social/tags/probably" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>probably</span></a> <a href="https://mastodon.social/tags/limitations" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>limitations</span></a> <a href="https://mastodon.social/tags/adequately" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>adequately</span></a> <a href="https://mastodon.social/tags/irregularities" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>irregularities</span></a> <a href="https://mastodon.social/tags/proportions" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>proportions</span></a></p>
Dr Mircea Zloteanu ☀️ 🌊🌴<p><a href="https://mastodon.social/tags/statstab" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>statstab</span></a> #395 The n-of-1 randomized controlled trial: clinical usefulness</p><p>Thoughts: Could RCTs comprised of 1 participant be useful? Maybe! And more so than large sample observational studies.</p><p><a href="https://mastodon.social/tags/Nof1" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Nof1</span></a> <a href="https://mastodon.social/tags/RCTs" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>RCTs</span></a> <a href="https://mastodon.social/tags/causalinference" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>causalinference</span></a> <a href="https://mastodon.social/tags/research" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>research</span></a> <a href="https://mastodon.social/tags/methods" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>methods</span></a> <a href="https://mastodon.social/tags/clinical" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>clinical</span></a></p><p><a href="https://pubmed.ncbi.nlm.nih.gov/2297206/" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">pubmed.ncbi.nlm.nih.gov/229720</span><span class="invisible">6/</span></a></p>
oSoTL Journal<p>Jo Ferrie, Thees Spreckelsen talk about "Teaching methods: Pedagogical challenges in moving beyond traditionally separate quantitative and qualitative classrooms" <a href="https://mastodon.social/tags/oSoTLSummer" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>oSoTLSummer</span></a> <a href="https://mastodon.social/tags/SoTL" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>SoTL</span></a> <a href="https://mastodon.social/tags/AcWri" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>AcWri</span></a> <a href="https://mastodon.social/tags/highered" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>highered</span></a> <a href="https://mastodon.social/tags/Vol2No2_22" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Vol2No2_22</span></a> <a href="https://mastodon.social/tags/SpecialEdition" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>SpecialEdition</span></a> <a href="https://mastodon.social/tags/methods" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>methods</span></a> <a href="https://mastodon.social/tags/TeachingMethods" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>TeachingMethods</span></a> <a href="https://mastodon.social/tags/SocialResearchMethods" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>SocialResearchMethods</span></a> <a href="https://osotl.org/osotl/article/view/64/58" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">osotl.org/osotl/article/view/6</span><span class="invisible">4/58</span></a></p>
News Beep<p>Surge in house fire deaths blamed on alternative heating methods</p><p>“In order to reduce the cost of living and reduce their use of electricity, they’re resorting to using…<br><a href="https://newsbeep.org/tags/NewsBeep" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>NewsBeep</span></a> <a href="https://newsbeep.org/tags/News" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>News</span></a> <a href="https://newsbeep.org/tags/Headlines" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Headlines</span></a> <a href="https://newsbeep.org/tags/alternative" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>alternative</span></a> <a href="https://newsbeep.org/tags/blamed" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>blamed</span></a> <a href="https://newsbeep.org/tags/deaths" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>deaths</span></a> <a href="https://newsbeep.org/tags/fire" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>fire</span></a> <a href="https://newsbeep.org/tags/heating" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>heating</span></a> <a href="https://newsbeep.org/tags/house" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>house</span></a> <a href="https://newsbeep.org/tags/in" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>in</span></a> <a href="https://newsbeep.org/tags/methods" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>methods</span></a> <a href="https://newsbeep.org/tags/midday" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>midday</span></a> <a href="https://newsbeep.org/tags/NewZealand" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>NewZealand</span></a> <a href="https://newsbeep.org/tags/NZ" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>NZ</span></a> <a href="https://newsbeep.org/tags/on" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>on</span></a> <a href="https://newsbeep.org/tags/Report" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>Report</span></a> <a href="https://newsbeep.org/tags/surge" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>surge</span></a><br><a href="https://www.newsbeep.com/18545/" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://www.</span><span class="">newsbeep.com/18545/</span><span class="invisible"></span></a></p>
$: Iam_jfnklstrm<p>I just love how easy it is to code interviews and meeting notes in obsidian.md using just three community plugins <a href="https://social.linux.pizza/tags/science" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>science</span></a> <a href="https://social.linux.pizza/tags/methods" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>methods</span></a> <a href="https://social.linux.pizza/tags/coding" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>coding</span></a> <a href="https://social.linux.pizza/tags/software" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>software</span></a> <a href="https://social.linux.pizza/tags/obsidian" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>obsidian</span></a></p>
N-gated Hacker News<p>🤔 Oh, the audacity! 67 pages and 50 figures to tell us that the old <a href="https://mastodon.social/tags/methods" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>methods</span></a> of simulating the <a href="https://mastodon.social/tags/universe" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>universe</span></a> are, gasp, <a href="https://mastodon.social/tags/flawed" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>flawed</span></a>. 🚀📉 Here's a *new* scheme, because clearly, the cosmos was just waiting for a <a href="https://mastodon.social/tags/moving" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>moving</span></a> <a href="https://mastodon.social/tags/mesh" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>mesh</span></a> to finally make sense. 🙄<br><a href="https://wwwmpa.mpa-garching.mpg.de/~volker/arepo/" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">wwwmpa.mpa-garching.mpg.de/~vo</span><span class="invisible">lker/arepo/</span></a> <a href="https://mastodon.social/tags/audacity" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>audacity</span></a> <a href="https://mastodon.social/tags/simulation" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>simulation</span></a> <a href="https://mastodon.social/tags/new" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>new</span></a> <a href="https://mastodon.social/tags/HackerNews" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>HackerNews</span></a> <a href="https://mastodon.social/tags/ngated" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>ngated</span></a></p>
サファイア・ネオ<p>AI絵生成 Генерація зображень ШІ<br>AI絵生成ソフトで何処が失敗してるかと云えば、人間の体の構造や比率を充分学習させてないと云う点が大きい。<br>Основна помилка програмного забезпечення для створення зображень ШІ полягає в тому,</p><p><a href="https://note.com/poison_raika/n/n501ac31af2f1" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">note.com/poison_raika/n/n501ac</span><span class="invisible">31af2f1</span></a></p><p>&lt;&gt;</p><p><a href="https://mastodon.social/tags/AI" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>AI</span></a> <a href="https://mastodon.social/tags/3d" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>3d</span></a> <a href="https://mastodon.social/tags/image" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>image</span></a> <a href="https://mastodon.social/tags/model" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>model</span></a> <a href="https://mastodon.social/tags/new" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>new</span></a> <a href="https://mastodon.social/tags/human" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>human</span></a> <a href="https://mastodon.social/tags/software" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>software</span></a> <a href="https://mastodon.social/tags/animals" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>animals</span></a> <a href="https://mastodon.social/tags/traditional" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>traditional</span></a> <a href="https://mastodon.social/tags/layout" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>layout</span></a> <a href="https://mastodon.social/tags/structure" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>structure</span></a> <a href="https://mastodon.social/tags/generation" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>generation</span></a> <a href="https://mastodon.social/tags/frequency" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>frequency</span></a> <a href="https://mastodon.social/tags/necessary" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>necessary</span></a> <a href="https://mastodon.social/tags/develop" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>develop</span></a> <a href="https://mastodon.social/tags/vizualize" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>vizualize</span></a> <a href="https://mastodon.social/tags/objects" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>objects</span></a> <a href="https://mastodon.social/tags/dimensions" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>dimensions</span></a> <a href="https://mastodon.social/tags/approach" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>approach</span></a> <a href="https://mastodon.social/tags/methods" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>methods</span></a> <a href="https://mastodon.social/tags/probably" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>probably</span></a> <a href="https://mastodon.social/tags/limitations" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>limitations</span></a> <a href="https://mastodon.social/tags/adequately" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>adequately</span></a> <a href="https://mastodon.social/tags/irregularities" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>irregularities</span></a> <a href="https://mastodon.social/tags/proportions" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>proportions</span></a></p>
💧🌏 Greg Cocks<p>Awesome Strategies To Visualize Change With Time<br>--<br><a href="https://medium.com/@yuanbo.faith/awesome-strategies-to-visualize-change-with-time-f8a7fa8362f2" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://</span><span class="ellipsis">medium.com/@yuanbo.faith/aweso</span><span class="invisible">me-strategies-to-visualize-change-with-time-f8a7fa8362f2</span></a> &lt;-- shared technical article<br>--<br><a href="https://www.databrewer.co/R/gallery" rel="nofollow noopener" translate="no" target="_blank"><span class="invisible">https://www.</span><span class="">databrewer.co/R/gallery</span><span class="invisible"></span></a> &lt;-- shared further examples &amp; background/processes<br>--<br>“This article summarizes effective strategies to visualize temporal changes, illustrated with inspiring graphic examples (with link to source code [and methods])…”<br><a href="https://techhub.social/tags/GIS" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>GIS</span></a> <a href="https://techhub.social/tags/spatial" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>spatial</span></a> <a href="https://techhub.social/tags/mapping" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>mapping</span></a> <a href="https://techhub.social/tags/datavisualisation" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>datavisualisation</span></a> <a href="https://techhub.social/tags/visualisation" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>visualisation</span></a> <a href="https://techhub.social/tags/R" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>R</span></a> <a href="https://techhub.social/tags/code" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>code</span></a> <a href="https://techhub.social/tags/methods" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>methods</span></a> <a href="https://techhub.social/tags/scripts" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>scripts</span></a> <a href="https://techhub.social/tags/opensource" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>opensource</span></a> <a href="https://techhub.social/tags/spatiotemporal" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>spatiotemporal</span></a> <a href="https://techhub.social/tags/temporal" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>temporal</span></a> <a href="https://techhub.social/tags/temporalchange" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>temporalchange</span></a> <a href="https://techhub.social/tags/visualise" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>visualise</span></a> <a href="https://techhub.social/tags/graphic" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>graphic</span></a> <a href="https://techhub.social/tags/examples" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>examples</span></a> <a href="https://techhub.social/tags/opendata" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>opendata</span></a></p>

"Publishing #diamond #OpenAccess is a noblesse oblige for us. Let everyone benefit from research, so that we contribute together to appropriate care and improve the quality of life for people with #intellectual #disabilities."

Read the interview with Alain Dekker, editor of an open access book on #psychosocial #support #methods:

🔗 rug.nl/library/open-access/blo

The book, published in 2024, has been downloaded >10,000 times by now.

#research #care #psychology #SocialWork

📷 by Silvio Zangarini

14 Ways to Quickly Improve Your Photography fro, Digital Photography School [Shared]

Feeling like your photography skills have plateaued? It happens to the best of us. One minute, you’re cruising along, picking up new techniques and elevating your sense of composition and light like a boss – and the next, you’ve hit a creative wall.

But don’t worry! While there’s no single magic bullet for improving your photography, I do have plenty of techniques and exercises that are designed to help you level up your skills, and that’s what I share in this article.

Note that different techniques will work better for different shooters, so if you don’t like a method, just skip it and move on. With any luck, you’ll find an approach that works for you, and you’ll be able to develop that creative eye once again.

welchwrite.com/blog/2025/03/26

Here's a #Ruby #design #question that regularly pops up in various contexts such as this: The Mailjet gem is a REST API adapter, 99% generated code. It exposes things as #class #methods like `Mailjet::Contact.create`. For a simpler, more consistent interface, I'm wrapping these in my own #PORO service class, all class methods as well, but I don't like this orgy of `class << self`. How to better design this? Here's a gist with the method bodies removed for readability: gist.github.com/svoop/25accb41

Gistwebhook_service.rbGitHub Gist: instantly share code, notes, and snippets.

and this paper also in MBMG erally nice : good work Tiina 😀
mbmg.pensoft.net/article/13083
Technology Readiness Level of biodiversity monitoring with molecular methods – where are we on the road to routine implementation?
by Laamanen et al.
#edna #methods #TRL #metabarcoding #standardisation

Metabarcoding and MetagenomicsTechnology Readiness Level of biodiversity monitoring with molecular methods – where are we on the road to routine implementation?Human activities are causing rapid biodiversity loss across ecosystems, affecting human well-being and crucial ecosystem services. Traditional biodiversity monitoring tools cannot keep up with the increasing demands of monitoring due to their limited spatial or temporal coverage, high costs, and lack of taxonomic expertise. Thus, implementation of novel molecular monitoring methods such as environmental DNA (eDNA) and DNA metabarcoding, are necessary. Molecular monitoring methods offer significant benefits for biodiversity monitoring and environmental assessment: high sensitivity and accuracy, non-invasive sampling, broad taxonomic range and cost and time efficiency. However, the diverse methodological approaches lead to poor comparability between studies and surveys, highlighting the need for standardised assessments. We used the Technology Readiness Level (TRL) framework to evaluate the maturity of molecular monitoring methods, providing a structured assessment of their readiness for routine use. In a systematic literature review, 420 articles fulfilling the study criteria were assessed and both individual studies and method categories ranked according to the TRL scale. The findings revealed a growing number of studies, particularly in aquatic environments, with most studies validating molecular technologies on a small scale but lacking large-scale system demonstrations. Aquatic eDNA-based methods targeting fish showed overall higher technology readiness compared to other sample types and taxa and applications of molecular monitoring methods ranked into the highest TRL were predominantly freshwater studies. Key barriers to the broader implementation of molecular methods to monitoring include the need for international standards, better quantitative estimates and comprehensive reference libraries. National and international cooperation is crucial for establishing common standards, ensuring reliable and comparable results and expediting the routine use of molecular methods in biodiversity monitoring. Recent efforts towards international standardisation are encouraging, but further coordinated actions are necessary for the global implementation and acceptance of these methods.

Nice paper in MBMG about eDNA methods standardisation
mbmg.pensoft.net/article/13207
What is eDNA method standardisation and why do we need it? by Theroux et al.
#Environmental #DNA #lab #accreditation #management #methods #standardisation #edna

Metabarcoding and MetagenomicsWhat is eDNA method standardisation and why do we need it?The rapid advancement of environmental DNA (eDNA) science in the past two decades has inspired a concomitant growth in the development of eDNA sampling and analytical methods. However, these methods are often developed by individual laboratories or institutions, which can isolate protocols within programmes, agencies or regions and prevent the beneficial exchange of data and ideas. Recent efforts to advance national and international coordination have resulted in a groundswell of standardisation efforts, but there is still considerable confusion around the role of formal standards for regulatory or research applications. With this commentary, we hope to provide clarity on the terminology used in standardisation discussions, including the differences between formal standards and best practice guidelines. Additionally, we discuss how eDNA method choice may be informed by environmental management scenarios and review examples of formal eDNA method standards being used to inform management action. The eDNA community now has an opportunity to develop a roadmap for method development to help close standardisation gaps, advance eDNA method adoption and accelerate our ability to monitor biological life at the scales our current environmental challenges demand.

Happily joining today University of Frankfurt am Main (aka Goethe Uni, GU) for a position to teach #anthropological #theory, #ethnographic #methods & #ScienceAndTechnologyStudies. My new academic base is GU's Institute of Cultural Anthropology & European Ethnology, @KAEEGoetheUni.

Fun plan: use this base to continue exploring theoretical roots of #STS in #sociology, #anthro, engage with #geography takes on STS. Meanwhile continuing research with #MaterialSemiotics. #introduction 4 assemblage.es

Here's my introduction post!

I'm a #survey methodologist and professor of #sociology at the University of Nebraska-Lincoln. I also direct our academic survey center, the Bureau of Sociological Research. And I edit the survey methods side of the Journal of Survey Statistics and Methodology.

My work is on mixed-mode surveys, interviewers, qnaire design, and nonresponse.

I will post about survey #methods work.