PsychoticSheep<a href="https://pixelfed.social/discover/tags/SyntheticBiology?src=hash" class="u-url hashtag" rel="nofollow noopener" target="_blank">#SyntheticBiology</a> <a href="https://pixelfed.social/discover/tags/GeneticEngineering?src=hash" class="u-url hashtag" rel="nofollow noopener" target="_blank">#GeneticEngineering</a> <a href="https://pixelfed.social/discover/tags/Microbiology?src=hash" class="u-url hashtag" rel="nofollow noopener" target="_blank">#Microbiology</a> <a href="https://pixelfed.social/discover/tags/LaboratoryResearch?src=hash" class="u-url hashtag" rel="nofollow noopener" target="_blank">#LaboratoryResearch</a> <a href="https://pixelfed.social/discover/tags/BScThesis?src=hash" class="u-url hashtag" rel="nofollow noopener" target="_blank">#BScThesis</a><br> Hey, just a quick real-life post! This post is all about the experimental workflow in my bachelor's thesis. From genetic modification to analysis, the key steps of the project are shown here. Each picture represents an important part of the process—starting with experimental design and lab work in molecular biology, followed by verification steps like PCR, gel electrophoresis, and HPLC.<br> <br> The goal? Boosting 2-butanol production in Acetobacterium woodii by swapping the adh4 promoter. Let's dive into the details!"<br> <br> Pic 1: Draft of my BSc thesis<br> Pic 2: The molecular biology lab<br> Pic 3: PCR for the amplification of RHA, LHA, and the promoters<br> Pic 4: Gel electrophoresis confirming the successful amplification of RHA, LHA, pta, and rso5<br> Pic 5: E. coli colonies carrying RHA, LHA, and the promoters (pta, rso5)<br> Pic 6: Gel electrophoresis to verify successful transformation of the promoters into E. coli – pta successful, rso5 failed<br> Pic 7: Preparing cryo stocks for the spacer2 and spacer2_pta E. coli strains<br> Pic 8: Midiprep of plasmid DNA from E. coli<br> Pic 9: White pellet: the extracted plasmid DNA<br> Pic 10: NanoDrop measurement of the plasmid DNA<br> Pic 11: Transformation of plasmid DNA into A. woodii under nitrogen conditions to ensure an anaerobic environment.<br> Pic 12: Gel electrophoresis confirming the successful promoter exchange in A. woodii<br> Pic 13: Screenshot from CLC Genomics Workbench: The pta promoter was amplified from A. woodii and sequenced, and the new promoter was successfully integrated<br> Pic 14: Testing ADH expression under the new promoter (Optical Density at 600/660 nm and HPLC)<br> Pic 15: Preparing high-performance liquid chromatography (HPLC) samples<br> Pic 16: The HPLC setup<br> Pic 17: Analysis of chromatograms in Chromeleon