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#reference

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Spotted on Mastodon: a spreadsheet of 900 media outlets with a Fediverse presence. “I did it! Although it’s still very rough & really not pretty, I got my spreadsheet with (> 900) verified accounts of media organizations in the #Fediverse away from #Google. The solution was #DataTables all along.”

https://rbfirehose.com/2025/04/09/spotted-on-mastodon-a-spreadsheet-of-over-900-media-outlets-with-a-fediverse-presence/

ResearchBuzz: Firehose | Individual posts from ResearchBuzz · Spotted on Mastodon: A Spreadsheet of Over 900 Media Outlets With a Fediverse Presence | ResearchBuzz: Firehose

Printed myself a 68xx #reference guide and put it in a binder. It'll be nice having a paper reference while I'm working on the Time Machine #Pinball #disassembly effort.

I've really been enjoying having a color #printer of my very own, and I'm especially happy about the fact that I haven't even put a visible dent in the ink tank fill indicators from the first fillup. The #EcoTank was pricey, but not having to be skimpy on ink usage is worth it.

Happy to announce a new paper of my 0hd student Paula Gauvin:
An annotated reference library for supporting DNA metabarcoding analysis of aquatic macroinvertebrates in French freshwater environments
mbmg.pensoft.net/article/13777
#metabarcoding #eDNA #reference #library @pensoft @mbmg

Metabarcoding and MetagenomicsAn annotated reference library for supporting DNA metabarcoding analysis of aquatic macroinvertebrates in French freshwater environmentsFreshwater ecosystems are increasingly threatened by human activities, leading to biodiversity loss and ecosystem degradation. Effective biodiversity monitoring, particularly through the use of aquatic macroinvertebrates as bioindicators, is crucial for assessing ecological health. While traditional morphological methods face limitations, DNA metabarcoding offers higher accuracy and efficiency in species identification using environmental DNA. However, the success of metabarcoding is contingent on the quality of reference libraries, which are often incomplete or biased. This study aimed to construct and share a comprehensive COI-based DNA barcode library for freshwater macroinvertebrates in France, specifically targeting short gene regions amplified with fwhF2/fwhR2N primers, suitable for degraded DNA. A list of species occurring in French freshwater ecosystems was established from official national checklists and Alpine lake surveys. The resulting library was analysed for taxonomic completeness, barcode coverage and cryptic diversity. The checklist consisted of 2,841 species across 10 phyla, for which 56% had at least one COI-5P sequence available in the Barcode of Life Data System (BOLD). The analysis of cryptic diversity, based on Barcode Index Numbers (BINs) highlighted a potential high rate of cryptic diversity, although it might have been overestimated due to the wide geographic origin of the sequences. Alignment challenges with the primers were identified for certain taxa, particularly amongst Coleoptera, Diptera and Malacostraca. The genetic diversity approached by the number of haplotypes per species highlighted that most of the species have limited diversity, with only three species having more than 100 haplotypes. Finally, this study showed that a total of 57 haplotypes were shared amongst 116 distinct species. This work emphasises the need for expanded sequencing efforts to improve barcode coverage and highlighted the pitfalls associated with the use of these primers for further biodiversity assessment of macroinvertebrates with DNA.
Replied to cob

Une relation n’est pas un rapport de force mais un rapport d’égalité 🤝

Savoir dire « #non » c’est savoir se dire « #oui » à soi-même.
✔️ Un « non » reste un « non ». Il n’y a pas de demi-consentement.

Le #porno véhicule parfois l’image d’un rapport de force, d’un consentement ambigu.
Mais il ne doit pas y avoir d’ambiguïté. ❌
Un rapport, non-consenti, c’est NON ! 🙅🏾‍♂️

✔️ Etre excité physiquement n’implique pas consentement.

Replied to cob

Demander la permission ce n'est pas la honte, bien au contraire ! 👍
On peut parfois penser que demander l'accord explicite de la personne risque de rendre la situation embarrassante et on ne sait pas vraiment comment le faire. 😳
Petit tips: les phrases les plus simples sont toujours les meilleures.

Replied to cob

Non, les #rapports ne sont pas improvisés ! 😮

Dans les films #porno, les gestes sont souvent prévus au millimètre près dans des studios cachant l'envers du décor et ne laissant pas de place à la spontanéité. Il arrive aussi que les femmes soient forcées de réaliser ces relations, contre leur volonté.

Les scénarios n'ont rien à voir avec la réalité et proposent des situations qui n'arrivent pas dans la vraie vie.